Fig. 1.

Effects of AS on 2DG uptake. L929 fibroblast cells were incubated at 37 °C for 30 min in DMEM media (5.5 mM glucose) with or without 10.0 mM AS. Ten-minute 2DG uptakes were then measured as described in materials and methods in the presence and absence of 10 mM AS. Con (control) represents cells not exposed to AS, (Tr) represent cells exposed with AS during the 30-min treatment phase, (Tr + Up) represents cells treated to AS during both the treatment and uptake phase, and (Up) represents cells exposed to AS only during the 10-min uptake phase. Data are means ± S.E from four wells. ^aSignificantly greater than control and ^bsignificantly different than maximum effect (Up) at P < 0.01.