Table 2.
[AS] | Control | +Azide | +Berb | +No glucose |
---|---|---|---|---|
0 mM | 0.59 ± 0.03 | 1.88 ± 0.06* | 1.74 ± 0.08* | 2.82 ± 0.07* |
5.0 mM | 1.83 ± 0.09# | 2.21 ± 0.09 | 1.88 ± 0.04 | 3.00 ± 0.10 |
L929 fibroblast cells were incubated for 30 minutes at 37° in DMEM media containing either 0 mM glucose (no glucose), or 5.5 mM glucose containing no additions (control), 5.0 mM sodium azide, or 50 μM berberine. Glucose uptake was measured as described in materials and methods in the presence and absence of 5.0 mM AS. Data are the means from quadruplicate samples of 2DG uptake in nmol/10 min/well ± standard error. Significance at P ± 0.01 from 0 mM AS control (*), or from respective conditions without AS (#).