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. Author manuscript; available in PMC: 2016 Aug 26.
Published in final edited form as: Hypertension. 2013 Jan 2;61(3):690–700. doi: 10.1161/HYPERTENSIONAHA.111.00318

Figure 2.

Figure 2

MALDI spectra peak areas from mouse podocytes, incubated with Ang(2-10) indicate production of Ang(3-10), Ang(4-10), Ang(5-10) and Ang(2-7). Ang(2-10) label is inside a text box to indicate that it was the added substrate. Control cases were sampled just following substrate addition (TP0) and 1, 2, 4, and 6 hours post-addition (TP0, 1H, 2H, 4H, 6H). In the absence of cells, the peptide levels were stable over the 6 hour incubation period. Peak areas were normalized to total angiotensin peptide current and bars show mean of three experiments and one standard deviation about the mean. No evidence of time dependent decay of Ang(2-10), production of other fragments, or post-source decay is evident in the cell-free cases.