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. Author manuscript; available in PMC: 2016 Aug 26.
Published in final edited form as: Hypertension. 2013 Jan 2;61(3):690–700. doi: 10.1161/HYPERTENSIONAHA.111.00318

Figure 4.

Figure 4

Ang peptide quantitation from mouse podocytes, incubated with Ang(2-10) in confirmatory experiments support production of Ang(3-10), Ang(4-10), Ang(5-10) and Ang(2-7), decreased Ang(2-7) production under NEP inhibition, and decreased production of Ang(3-10) under APA inhibition. Ang(2-10) label is inside a text box to indicate that it was the added substrate. Cells alone or following pretreatment with SCH39370 (SCH, NEP inhibitor) or amastatin (AM, APA inhibitor) were sampled immediately following substrate addition (TP0) and 2 and 4 hours post addition (2H, 4H). Ang(2-10), Ang(3-10), and Ang(2-7) were quantified using AQUA peptides, Ang(4-10) and Ang(5-10) were normalized to the sum of the AQUA peptide areas. Bars show the mean of three experiments and one standard deviation about the mean.