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. 2016 Jun 9;65(9):2810–2815. doi: 10.2337/db15-1666

Figure 3.

Figure 3

The DNA binding of wild-type PTF1A and PTF1A-P191T as part of the PTF1A:E12 dimer or PTF1A:E12:RBPJ trimer in EMSA. The relative abilities of wild-type and p.P191T mutant PTF1A to form complexes with E12 and RBPJ on the PTF1 binding site of the Rbpj1 gene was tested in vitro. Lanes 3a and 4a show lanes 3 and 4 with an expanded grayscale such that the PTF1 trimeric complex bands are not saturated. PTF1A alone does not bind DNA. 2, PTF1A:E12 dimer; 3, PTF1A:E12:RBPJ trimer; d, E12 homodimer; J, RBPJ monomer; M, p.P191T mutant; W, PTF1A wild type.