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. 2016 Aug 24;6:32174. doi: 10.1038/srep32174

Figure 2. VV-miR-34a and VV-Smac synergistically induce apoptosis through activation of the caspase pathway in MM cells.

Figure 2

(A) Three MM cell lines, QSG-7701 and human PBMCs were infected with VV, VV-miR-34a, VV-Smac and VV-miR-34a combined with VV-Smac at the MOI of 1, 2, 4, and 8. 72 hours later, cell viability rate was measured by MTT assay. The results were presented as the mean ± SD (n = 6) of three independent experiments. *represents P < 0.05, ** represents P < 0.005. (B) RPMI-8226 cells were treated with the indicated OVVs at 4 MOI. After 48 hours, whole-cell lysates were subjected to western blotting to assess the cleavage of caspase-9, -3 and PARP. (C) Annexin V/PI-staining method was used to detect the apoptosis induced by the indicated viruses at the MOI of 4 for 48 hours. (D) RPMI-8226 cells were pretreated with Z-LEHD-FMK (40uM) for 4 hours, and then infected with the indicated viruses for 48 hours at 4 MOI. Whole-cell lysates were analyzed for inhibition of activated caspase-9 by western blot (a) and apoptotic proportion by flow cytometry (b). Full length blots were shown in Fig. S1.