Germaria of (A) control, (D) Wnt4C1/Df(2L)BSC291 and (G) c587-gal4/+; UAS-sgg/+ stained for Hts (green) and Nos (red). (B) Control, (E) Wnt4C1/Df(2L)BSC291 and (H) c587-gal4/+; UAS-sgg/+ germaria stained for α-Spectrin (green) and Sxl (red). (C) Control, (F) Wnt4C1/Df(2L)BSC291 and (I) c587-gal4/+; UAS-sgg/+ stained for α–Spectrin (green) and Bam (red). The GSC-like cells that accumulate upon Wnt pathway disruption express Nos and Sxl, but not the differentiation factor Bam. (Scale bars, 10 μm).