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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Dev Biol. 2016 Jun 27;417(1):50–62. doi: 10.1016/j.ydbio.2016.06.038

Fig. 4. The canonical Wnt pathway functions during adulthood to promote germ cell differentiation.

Fig. 4

(A–D) Germaria from c587-Gal4/+; UAS-arm-RNAi/+ females raised at (A) 18°C, (B) 29°C, (C) shifted from 18°C to 29°C upon eclosion or (D) shifted from 29°C to 18°C upon eclosion stained for Vasa (green), Hts (red) and DNA (blue). Knockdown of arm in adulthood led to the formation of tumors containing more than 15 round fusomes (B and C) while knockdown of arm only during development led to the formation of smaller tumors (D). (E) A graph showing the percent of germaria (Y axis) that contain more (black bars) or less (light gray) than 15 round fusomes per germarium in c587-gal4/+; UAS-armRNAi/+ females. (F) Graph showing the percentage of ovarioles that contain the indicated numbers of maturing egg chambers under each of the conditions. (Scale bars, 10 μm).