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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Dev Biol. 2016 Jun 27;417(1):50–62. doi: 10.1016/j.ydbio.2016.06.038

Fig. 5. Canonical Wnt pathway limits Dpp signaling within the germarium.

Fig. 5

(A) Control, (B) Wnt4C1/Df(2L)BSC291, (C) c587-gal4/+; UAS-dshRNAi/+ and (D) c587-gal4/+; UAS-sgg/+ ovaries carrying a Dad-LacZ enhancer trap stained for Hts (green), LacZ (red) and DNA (blue). RNA in situ hybridization of dpp and Hts immunostaning were performed on (E) control, (F) Wnt4C1/Df(2L)BSC291, (G) c587-gal4/+; UAS-dshRNAi/+ and (H) c587-gal4/+; UAS-sgg/+ ovaries. Arrows point to cells expressing dpp mRNA. (E) In control germaria, dpp transcripts were mostly detected in the cap cells. (F-H) Disruption of the canonical Wnt pathway led to an upregulation of dpp transcripts in the escort cells. RNAi knockdown of dpp partially rescued the tumorous phenotype of (I) Wnt4C1/Df(2L)BSC291 and (J) c587-gal4/+; UAS-dshRNAi/+ germaria. (K) Quantification of the suppression of Wnt pathway mutants phenotypes by RNAi knockdown of dpp. (Scale bars, 10 μm).