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Validation of gene knockdown. A, B) qPCR (A) and Western blot (B) analyses showed that siRNA treatments for 72 h reduced DNMT expression in embryonic cardiomyocytes. C) Time course of Dnmt mRNA expression after siRNA transfection (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 vs. negative siRNA.
