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Knockdown of DNMT3a expression alters sarcomere assembly in embryonic cardiomyocytes. At 72 h after negative control (A), DNMT1 (B), DNMT3a (C), or DNMT3b (D) siRNA transfection, cardiomyocytes were stained with antisarcomeric α-actinin antibody (sarcomere, green) and DAPI (nucleus, blue). Disarray of sarcomere was observed in the cardiomyocytes treated with DNMT3a siRNA (C). Scale bar, 10 μm.
