Figure 3.

Knockdown of DNMT3a expression affects the survival of embryonic cardiomyocytes. A–F) Cell viability at 24 (A) and 72 h (D) after siRNA transfection; cell viability at 24 (B) and 72 h (E); and apoptosis at 24 (C) and 72 h (F) were measured with an ApoTox-Glo Triplex Assay. G) At 72 h, the cardiomyocytes transfected with DNMT3a siRNA or negative siRNA were stained with α-actinin (green) and DAPI (blue) and imaged by a multimode plate reader. H, I) The percentage of cardiomyocytes (α-actinin⁺ cells/DAPI⁺ cells) (H) and total area of cardiomyocytes (α-actinin⁺ area) (I) were decreased in DNMT3a siRNA-treated cultures. (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 vs. negative siRNA.