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. 2016 Jun 15;30(9):3238–3255. doi: 10.1096/fj.201600346R

Figure 4.

Figure 4.

Knockdown of DNMT3a expression decreases the beating rate of embryonic cardiomyocytes. A) Counting cell contractions under a phase-contrast microscope, we found that transient knockdown of DNMT3a expression (12 nM siRNA) led to dramatic and rapid decline of beating rate. The beating rate recovered over time as the knockdown effect subsided. B) The inhibitory effect of DNMT3a siRNA was concentration dependent (low DNMT3a siRNA, 3 nM; medium DNMT3a siRNA, 6 nM; high DNMT3a siRNA, 12 nM; n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 vs. negative siRNA.