Figure 5.

Knockdown of DNMT3a expression inhibits the contractility of embryonic cardiomyocytes. The results from a video-based imaging assay revealed that the mean beating frequency (A), mean movement (px: pixel; B), maximum cell contraction velocity (C), maximum relaxation velocity (D), percentage of regions that move at contraction (E), and synchronicity score (F) for beating in the interrogated region were decreased in the cardiomyocytes treated with DNMT3a siRNA compared to negative-siRNA–treated cardiomyocytes (n = 3 wells, 2 imaged fields per well). *P < 0.05, **P < 0.01, ***P < 0.001 vs. negative siRNA.