TABLE 1.
Primers used for multiplex sodium bisulfite sequencing
| Gene and ID | Primer, 5′–3′ |
CG sites (n) | Location | |
|---|---|---|---|---|
| Forward | Reverse | |||
| Myh6 (17888) | AATAATTGAGGTAAGGGTTTGG | ACTTCCAAAAAAAACAATTTCC | 4 | Across TSS |
| Myh6 (17888) | TTTTTTATTAGGAGTGGAGGGT | ATCCTCCAAAAACCTTACTACC | 3 | Promoter |
| Myh7 (140781) | GTTTAGAGTTGTGTTTGGGAAG | CAACAAATAAAAAATTAATCCCAA | 9 | Promoter |
| Myh7 (140781) | AGGGTTAGGTTAGTATTGGGGG | CCCAATCCCTAACCAAATTTAA | 5 | Promoter |
| Myh7b (668940) | TTAGGGGTTGAGTTAGAGGAGA | CCTCCAAACATAAAAACATCCT | 3 | Promoter |
| Myh7b (668940) | TTATGTTGGATTTTTTTTTGGA | CAAAACACAAAAAACAAATTCC | 4 | Promoter |
| Nppa (230899) | GATTTTTGTAGTTGAGGGTTTG | TCCTAACCCTACCTTAAAATCC | 11 | Across TSS |
| Nppa (230899) | TTGTTTGGGGTTAGAGGTTTAT | CCTTAAACAACTCCACTCACC | 6 | Promoter |
| Nppb (18158) | TGGAAGGTGGGTATTAAAATTA | CCCTTCTTTCATACAAAACCTA | 10 | Promoter |
| Nppb (18158) | GTGTGTGTTTTTTTTGGGTTAG | CCCCACCATAAATAAAAAAAAA | 6 | Promoter |
| Tnnc1 (21924) | GAGTTTAAGGTGAGGAGGAGAT | AATCCTACCAAAAAACACTTCC | 6 | Promoter |
| Tnnc1 (21924) | GTTGGGTTTATAGTAGGGAAGG | TCCTCACCTTAAACTCTTACAAAA | 9 | Promoter |
| Tnni3 (21954) | TTTGGGTATGAATATGGGAAGT | TAAAATCTCCACCCCAAAACTA | 7 | Across TSS |
| Tnni3 (21954) | TTGAAGTAGGTTTAGGGATTGA | CTCCTATAAAATTCAACCAAACC | 7 | Promoter |
| Tnnt2 (21956) | GGGTTGGTGGATATAAATGTTTA | CAAAAATCAACCTCAAAAACAC | 6 | Promoter |
| Tnnt2 (21956) | GGGTTAGGGATTAAAGTTATTGG | CAACAAATCCCAACAAAATCTA | 9 | Promoter |
| Camta1 (100072) | GGGGTTGAGGTTAGGAAGG | AACCAATCAAAAAAAATTCC | 44 | Promoter |
| Camta1 (100072) | ATGTTGAGGAATTAAATAGTGTAAGTT | AACTCTCCTACCTATAAATCCACAC | 9 | Promoter |
| Camta2 (216874) | AGGATTTAGGATAATGAAGTTAGGTT | AACTAAAAACCCTAACTCTTACCTC | 34 | Across TSS |
| Camta2 (216874) | TAAGAAATTTGGAGAGAGTTTTG | AAAAAACACAAAACCAAATTCT | 8 | Promoter |
| Cdkn1A (12575) | TTAATGATGGAGTTGAGATTGG | CAAAACTAAACAAACCCAAACC | 5 | Promoter |
| Cdkn1A (12575) | TTTTTTTAGTGTTGGTTTGTGA | TACACACACCACACACATAAAC | 4 | Promoter |
| Cdkn1B (12576) | GAGATTTTTGGGGTTAGGTTAAG | ACCAACAAACCTACTCTAACTAACC | 28 | Promoter |
| Cdkn1B (12576) | AGTAATGGTTTTTATTGTGTTTAGG | CTAATTCAACCCACCTACATTC | 12 | Promoter |
| Cdkn1C (12577) | AGTTGGTGTAGTTTTAGGGTTAG | ATATAATATTTTCAATTTCAACAACAC | 55 | Promoter |
| Cdkn1C (12577) | TTAAGGTAGGTAGAATTGGGG | ACCATAAAACTAAACACAACCC | 14 | Promoter |
| Mef2c (17260) | AGAAATTATGATTGTTAAAGTGGA | AAAAAAATTAATCATACTCCCAA | 6 | Promoter |
| Mef2c (17260) | AGTTTGTGTGAAATGAGGAAATT | AAAACCCCCCAATTTAAACTAC | 7 | Across TSS |
| Mef2d (17261) | GGTTAAAGGTTTAGAAGTAATGGAG | AATAAACCCCAACTCCTACAC | 17 | Promoter |
| Mef2d (17261) | TGTTTTTTTTAGAAATAGTGGAAA | AAAACATCCAAAAACCTTATCT | 10 | Promoter |
TSS, transcription start site.