Figure 3.

Screening of specific gene networks reveals the TRPM8-dependence of genes encoding cold-shock proteins and antioxidant enzymes. A) Real-time PCR showed no significant variation in Trpm8 gene expression (exons 21–22) in CTL and KOM8 mice subjected to cold shocks of different amplitudes (scrotum temperatures were 29, 17, and 4°C). B–D) mRNA quantification was also performed for the following heat- and cold-shock factors (B): heat-shock factor 1 (Hsf1), cold-inducible RNA binding protein (Cirbp) and RNA binding motif (RNP1, RRM) protein 3 (Rbm3); for proliferation markers (C): antigen identified by monoclonal antibody Ki 67 (Mki67) and proliferating cell nuclear antigen (Pcna); inhibitors of cell cycle: cyclin-dependent kinase inhibitor 1A and 1B (Cdkn1a and Cdkn1b, respectively) also known as p21^cip1/waf1 and p27^kip; and for antioxidant enzymes (D): SOD1, Cu²⁺/Zn²⁺ (Sod1, Gpx4, and Gpx5, respectively) and UCP3 (Ucp3). Values are presented as means ± sd for CTL (n = 5) and KOM8 (n = 5) mice. Statistical significance confirmed with 1-way ANOVA when P < 0.05 and is indicated above the tested column as the number of the CTL column it is paired to.