TABLE 1.
Primers
| Gene | Forward (5–3′) | Reverse (5′–3′) |
|---|---|---|
| PCR | ||
| Trpm8(ex13–14) | TGAAGCTTCTGCTGGAGTGG | GAGTTCCACATCCAAGTCCTC |
| Trpm8 (ex16–19) | ATATGAGACCCGAGCAGTGG | CTGCCTCACTTCATCACAGAAG |
| Trpm8 (ex18–21) | CGAGACACGAAGAACTGGAAG | ATCCGTTGCAGAATTATAATCTGGG |
| Trpm8 (ex16–21) | ATATGAGACCCGAGCAGTGG | ATCCGTTGCAGAATTATAATCTGGG |
| β-Actin | CAGAGCAAGAGAGGTATCCT | GTTGAAGGTCTCAAACATGATC |
| Real-time PCR | ||
| Gapdh | CTGCGACTTCAACAGCAACTC | TCCACCACCCTGTTGCTGTA |
| Gdi2 | GGAATACGACGTGATCGTGC | CAGCGGTGTTATAGACGCACT |
| Ctdnep1 | ACAAACACCCAGTCCGGTTT | TGCCACAGCAGAGCCATAAA |
| Trpm8 | CACATATGACTTCTCCCACTGT | AGAGCATGTAGATGCACACCA |
| Magea4 | GGGAGTTAGACAATGTTCAGGCT | GGAGAGGAGGCTCTTTGAGG |
| Hspa2 (variant 1) | CGCTTTCGTCCTAACGTTGC | CGATGATCTCCACCTTGCCA |
| Tnp1 | CTCACAAGGGCGTCAAGAGA | CATTGCCGCATCACAAGTGG |
| Prm2 | CATAGGATCCACAAGAGGCG | TGCCTCCTACATTTCCTGCAC |
| Mki67 | TCTTGGCACTCACAGCCCAGC | TGTCCTCGGTGGCGTTATCCC |
| Pcna | TCTGCAAGTGGAGAGCTTGGCA | AGAGCAAACGTTAGGTGAACAGGCT |
| Cdkn1a | CGGTGTCAGAGTCTAGGGGA | AGGATTGGACATGGTGCCTG |
| Cdkn1b | GCTGGGTTAGCGGAGCAGTGT | AGCGTTCGGGGAACCGTCTG |
| Mtor | GCATTCCGACCGTCCGCCTT | CTGGAACGCCGCAGTCCGTT |
| Sp1 | GTGCCGCCTTTTCTCAGACT | CAATTCTGCTGCAGGTTGCT |
| Ddit3 | TGCAGGAGGTCCTGTCCTCAGAT | AGCCAAGCTAGGGACGCAGG |
| Atf4 | CATGGGTTCTCCAGCGACAA | TCCAACATCCAATCTGTCCCG |
| Eif2ak3 | CAAGCCAGAGGTGTTTGGGA | AGATTCGAGCAGGGACTCCA |
| SigmaR1 | AGGGCACCACGAAAAGTGAGGT | GGTCCCCACTCCAGAGCCGT |
| Hsf1 | GCCTCCCCAGGCAGGAGCATA | AGGGCTCGCCTCCAGTACCC |
| Hsf2 | CATCACCTGGAGTCAGAATGGA | GCACTACTTTTCGGAAGCCA |
| Cirbp | GGAGCTCGGGAGGGTCCTACA | GACGATCTGGACGCGGAGGG |
| Hspa1a | TGGCCTTGAGGACTGTCATT | AGCCCACGTGCAATACACAA |
| Rbm3 | CGTGGTCGCAGTTACTCTAG | TGAGTAGCGGTCATAGCCAC |
| Ucp1 | AGGAGTCCGAAGTCGCGGGT | TGGAGGGCAGAGAGGCGTGA |
| Ucp2 | TCTGCACCACCGTCATCGCC | GACCTGCGCTGTGGTACTGGC |
| Ucp3 | CCGAAGTGCCTCCCACAACGG | ACGGACCTTGGCGGTGTCCA |
| Sod1 | GCGGTGAACCAGTTGTGTTG | GCACTGGTACAGCCTTGTGT |
| Sod2 | ACAACTCAGGTCGCTCTTCAG | TCCAGCAACTCTCCTTTGGG |
| Sod3 | CTGACAGGTGCAGAGAACCTC | GGTCAAGCCTGTCTGCTAGG |
| Cat | GCCAATGGCAATTACCCGTC | GAGGCCAAACCTTGGTCAGA |
| Gpx1 | TCTCTCTGAGGCACCACGAT | CATTCTCCTGGTGTCCGAACT |
| Gpx2 | CTGCAATGTCGCTTTCCCAG | CCCCAGGTCGGACATACTTG |
| Gpx3 | GCATCCTGCCTTCTGTCCC | CGATGGTGAGGGCTCCATAC |
| Gpx4 | GTACTGCAACAGCTCCGAGT | ATGCACACGAAACCCCTGTA |
| Gpx5 | TGTGAAAGGCACCATCTACG | GACCGCAATAGGTAGCCACA |