TABLE 2.

The TRPM8-dependence of gene expression in mouse germ cells subjected to cold shock with a 3 d recovery period

Markers	Gene	4°C	17°C	29°C
Germ cell types	Magea4	1.09	1.49	0.95
	Hspa2	0.88	1.05	0.64
	Tnp1	1.53	1.81	0.81
	Prm2	1.73	1.91	0.76
Cell cycle	Mki67	0.89	0.95	0.72
	Pcna	1.38	1.04	0.92
	Cdkn1a	0.65	1.14	0.81
	Cdkn1b	1.32	1.04	0.82
Cell signaling	Mtor	0.9	1.06	0.8
	Sp1	1.37	1.61	1.61
ER stress	Ddit3	1.57	1.28	0.83
	Atf4	1.62	1.26	0.67
	Eif2ak3	1.14	1.13	0.98
	Sigmar1	1.47	1.17	0.67
Cold/heat shock proteins	Hsf1	2.18a	1.34	0.89
	Hsf2	0.75	0.75	1.05
	Cirbp	2.16a	1.51	1.68
	Hspa1	0.47b	0.68	1.08
	Hspa2	0.88	1.05	0.64
	Rbm3	2.11	1.5	0.87
Mitochondrial uncoupling	Ucp1	—	—	—
	Ucp2	1.07	1.01	0.78
	Ucp3	2.98a	1.56	1.20
Oxidation	Sod1	2.08a	2.04	1.20
	Sod2	1.12	1.28	0.72
	Sod3	0.75	0.95	0.65
	Cat	1.10	1.37	1.04
	Gpx1	1.62	1.25	0.87
	Gpx2	2.01a	1.87	0.94
	Gpx3	1.22	1.06	0.68
	Gpx4	1.71a	3.47a	3.38a
	Gpx5	3.58a	1.14	0.52

TRPM8-mediated fold induction of gene expression. Values represent the ratio of averaged gene expression obtained by dividing CTL value by KOM8 value. Statistical analysis was achieved with 1-way ANOVA.

^aTRPM8-dependent induction.

^bTRPM8-dependent repression.