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. 2016 Apr 15;44(14):6676–6692. doi: 10.1093/nar/gkw252

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Cohesin and Pds5 co-localize at Mis4+/Rad21+ loci. (A) ChIP-qPCR analysis showing Rad21-Pk9 at selected CARs. No tag strain was used to assess the background levels. * P < 0.05, two-tailed, paired student's t-test for Rad21-Pk9 compared to background levels. Error bars represent SD, n = 3. (B) Cohesin enrichment at Mis4+/Rad21+ versus Rad21+ sites. Boxplots comparing sum of log2 Rad21 ChIP signal at Mis4+/Rad21+ versus Rad21+. P < 0.05, one-sided Wilcoxon Rank Sum test. (C) Pds5 enrichment at Mis4+/Rad21+ versus Rad21+ sites. Bar graph shows distances between Pds5 peak margins at Mis4+/Rad21+ and Rad21+ sites in 5000 nt bins. An overlap between intervals spanned by a Mis4+/Rad21+ and Pds5 peak gives a distance of 0. (D) ChIP-qPCR analysis showing Pds5-GFP at selected CARs. No tag strain was used to assess the background levels. *P < 0.05, two-tailed, paired Student's t-test for Pds5-GFP compared to background levels. Error bars represent SD, n = 3.