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. 2016 Apr 15;44(14):6676–6692. doi: 10.1093/nar/gkw252

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Transcription induction mediates cohesin proteins recruitment to chromatin. (A) ChIP-qPCR analysis showing RNAPII enrichment at selected promoters after heat shock at 42°C, 30 min. Values are normalized to rpl29. Error bars represent SD, n = 3. *P<0.05, one-tailed, paired Student's t-test comparing 42 to 32°C values. (B) ChIP-qPCR analysis showing enrichment of RNAPII phosphorylated at Ser5 at selected promoters after heat shock at 42°C, 30 min. Values are normalized to act1. Error bars represent SD, n = 3. * P<0.05, two-tailed, paired student's t-test comparing 42°C to 32°C values. (C) RT-qPCR showing act1, hsp70 and hsp9 mRNA levels after heat shock. Error bars represent SD, n = 3. Values are normalized to 32°C signals. *P < 0.05, two-tailed, paired Student's t-test comparing 42 to 32°C values. (D) ChIP-qPCR analysis showing Psm3-GFP enrichment at hsp70 and hsp9 promoters after shifting from 32 to 42°C, 30 min. Values are normalized to rpl29. Error bars represent SD, n = 3. *P < 0.05, one-tailed, paired Student's t-test comparing 42 to 32°C values and GFP signal to no tag control. (E) ChIP-qPCR analysis showing Mis4-GFP enrichment at hsp70 and hsp9 promoters after shifting from 32 to 42°C, 30 min. Values are normalized to rpl29. Error bars represent SD, n = 3. *P < 0.05, one-tailed, paired Student's t-test comparing 42 to 32°C values and GFP signal to no tag control. (F) ChIP-qPCR analysis showing Eso1-GFP enrichment at hsp70 and hsp9 promoters after shifting from 32 to 42°C, 30 min. Values are normalized to rpl29. Error bars represent SD, n = 3. *P < 0.05, one-tailed, paired Student's t-test comparing 42 to 32°C values and GFP signal to no tag control.