Figure 4.

GcvB regulation of lrp mRNA through redundant pairing. (A) Schematic drawing of the secondary structure of GcvB. Regions R1, R2 and R3 have been shown to be involved in pairing with various mRNA targets (51–53,83). (B) Predicted base-pairing region of sRNA GcvB with the lrp sequences is shown. Mutated nucleotides are underlined and changes made to them are shown. (C) β-galactosidase activity from cells with lrp-lacZ (HL1071) and lrp_mt15-lacZ (HL1810) translational fusion plasmids, in the presence of plasmids that overexpress either wild-type pGcvB or pGcvB_mt8. Assayed as in Figure 2. Error bars indicate standard deviation.