Table 1. Sequence analysis of strand transfer products.
| # | Misincorporation | Untemplated | Sequence 5′-GCAUGGGCCC … -3′ |
|---|---|---|---|
| 24 | +C | AUGC C AUGC AUGC UUGC N0-3 | |
| 20 | +CC | AUGC CC AUGC AUGC UUGC N0-3 | |
| 2 | G→C | +C | AUCC C AUGC AUGC UUGC N0-3 |
| 38 | G→C | AUCC AUGC AUGC UUGC N0-3 | |
| 14 | (U/G)→C | ACC AUGC AUGC UUGC N0-3 | |
| 4 | +A(C/U)C | AUGC A(C/U)C AUGC AUGC UUGC | |
| 1 | AUGC AUGC UUGC | ||
| 1 | ΔG | AUC AUGC UUGC |
The first column indicates the total number of each sequence obtained, all of which were preceded with the core sym/sub sequence shown in the header line. Underlined nucleotides indicate misincorporations or untemplated additions (summarised in the indicated columns on the basis of the differences between the observed and expected [the latter highlighted in bold] sequences) at or near the junction of the sym/sub donor and acceptor sequences. N indicates the untemplated addition of any nucleotide, nucleotides in brackets indicate alternates, ΔG indicates a single nucleotide deletion.