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. 2016 Jun 28;44(14):6853–6867. doi: 10.1093/nar/gkw571

Figure 5.

Figure 5.

The P-TEFbs targeting NELF-A and NELF-E are recruited by distinct SEC subtypes. (A and B) The anti-Flag immunoprecipitates from NEs of HeLa cells with Cdk9-f cDNA and shRNA(s) co-transfection and HMBA treatment as indicated were analyzed by WB for the levels of indicated Cdk9-f/P-TEFb-associated proteins. (C and D) The anti-Flag immunoprecipitates derived from NEs of cells with indicated cDNA and shRNA(s) co-transfection and HMBA treatment were analyzed by WB for the levels of indicated proteins in association with Paf1c-f (C) or Med26-f (D). (E) LSEN of HeLa cells with indicated shRNA infection and HMBA treatment were analyzed by WB for the levels of indicated proteins. (F) ChIP-qPCR analysis for the enrichment of Pol II at the promoter region of representative genes in HeLa cells with indicated shRNA infection and HMBA treatment as in Figure 1E. The level in shGFP infected cells was set to 1.0. All values were expressed as Mean ± SD of three replicates. *P < 0.05, **P < 0.01; P-values were assessed using two-tailed Student's t-test.