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Modeling of the WT and G140S-Q148H (SH) IN active sites in complexes with the modified substrates. Models were generated for the WT and SH mutant intasomes carrying the native viral DNA sequence CAGT or the −1/+1 abasic derivatives. The positions of the two catalytic amino acids D64 and E152 are shown as sticks, and the positions of the two magnesium ions by spheres. Color coding and cation displacements (Mg B Shift, Å) compared to WT are tabulated.
