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. 2016 Jul 4;44(14):6625–6638. doi: 10.1093/nar/gkw600

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — CI repression of λ P_R activates P_RE by relief of TI. (A) Map of the λ CI-CII region. (B) Structure of the reporter constructs used to measure P_RE and P_R activity. (C) Activity of the CII-activated P_RE in the P_RE.(cro⁻ P_R).lacZ and P_RE.(cro⁻ P_R⁻).lacZ reporters with increasing CI repression of P_R. CI levels are expressed in wild-type lysogenic units (WLU), where 1 WLU is the amount of CI produced by a wild-type lambda lysogen. Activity is normalized to the highest LacZ units obtained in the P_RE.(cro⁻ P_R⁻).lacZ reporter (n = 9). (D) CI repression of P_R in the P_R.(cro⁻ P_RE). lacZ reporter in the presence or absence of P_RE activity (±CII). LacZ activities of each promoter were normalized by their value in the absence of CI (n = 9). Model fits in (C and D) were obtained by incorporation of λ-specific details (30) into the relief of pause-enhanced occlusion model of Figure 2 (see text).