Figure 5.

PTC readthrough at p53 R213X (TGA, TAG and TAA) sequences in cells and in vitro. (A) H1299 cells transiently transfected with the indicated constructs were exposed for 48 h to CDX5-1 and G418, alone or in combination and full-length and truncated p53 was quantified relative to the amount of p53 in untreated cells. p53-WT: R213; Mock: transfection reagents only. (B) PTC readthrough in a cell-free translation extract. The indicated 5′ capped and 3′ poly(A) tailed TP53 mRNAs were subjected to in vitro translation in the presence of the indicated compounds. Full-length p53 and truncated p53 were quantified relative to full-length p53 (in WT) or truncated p53 (in mutants) in untreated reactions.