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. 2016 Aug 22;9:5181–5193. doi: 10.2147/OTT.S105198

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© 2016 Xiao et al. This work is published and licensed by Dove Medical Press Limited

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Lv construction and the expression of miR-126.

Notes: (A) Agar gel electrophoresis: M, DL2000 DNA Marker; 1, 2, 3, and 4, PCR products (786 bp); (B) the expression of green fluorescent protein after 72 hours of virus transfection in 293T cells (×120), and the virus packaging efficiency (%) = the number of GFP expressed cells/cell count; (C) observation of the efficiency of virus-infected BLS cells (×120), the virus packaging efficiency (%) = the number of GFP expressed cells/cell count; (D) expression of miR-126 in bladder cancer BLS cells in each group. ** P<0.0001.

Abbreviations: GFP, green fluorescent protein; Lv, lentivirus; PCR, polymerase chain reaction.