Table 4. Genetic diversity in Enterocytozoon bieneusi based on the analysis of concatenated sequences from five genetic loci.
| Taxa | Test model | Variability of multilocus gene sequences | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| N | Hd | k | Pi | Theta (k) | Fs (Obs) | P (Fs ≤ Obs) | D (Obs) | P (D ≤ Obs) | LD (|D'|) | Rm | ||
| Changchun | F | 19 | 0.93 | 25 | 0.0117 | 25 | 2.14 | 0.830 | 2.19 | 0.994 | ||
| I | 16 | 0.91 | 13 | 0.0063 | 13 | 0.84 | 0.655 | 2.21 | 0.993 | 1.0024–0.1373X | 4 | |
| Daqing | F | 8 | 0.82 | 56 | 0.0263 | 56 | 19.23 | 1.000 | 1.16 | 0.908 | ||
| I | 7 | 0.75 | 11 | 0.0056 | 11 | 6.24 | 0.980 | 1.16 | 0.913 | 0.9635–0.1451X | 4 | |
| Harbin | F | 8 | 0.61 | 9 | 0.0041 | 9 | 4.86 | 0.961 | −1.12 | 0.121 | ||
| I | 6 | 0.37 | 3 | 0.0015 | 3 | 1.84 | 0.813 | −1.10 | 0.146 | 0.9988–0.1797X | 3 | |
| Qiqihar | F | 9 | 0.86 | 29 | 0.0138 | 29 | 5.66 | 0.993 | 1.61 | 0.977 | ||
| I | 8 | 0.85 | 10 | 0.0050 | 10 | 2.37 | 0.843 | 1.52 | 0.961 | 1.0282–0.2990X | 2 | |
| Total (4 cities) | F | 44 | 0.95 | 42 | 0.0197 | 42 | 3.26 | 0.835 | 2.10 | 0.978 | ||
| I | 37 | 0.93 | 13 | 0.0065 | 13 | −3.38 | 0.248 | 2.07 | 0.983 | 0.9352–0.2020X | 11 | |
| SP1 | F | 51 | 0.99 | 31 | 0.0142 | 31 | −10.35 | 0.025 | 0.04 | 0.583 | ||
| I | 33 | 0.97 | 6 | 0.0029 | 6 | −15.73 | 0.000 | 0.05 | 0.580 | 0.9473–0.1084X | 5 | |
| SP2 | F | 9 | 0.52 | 80 | 0.0388 | 80 | ∞ | N/A | −0.97 | 0.188 | ||
| I | 7 | 0.51 | 8 | 0.0051 | 8 | 7.68 | 0.983 | −0.92 | 0.175 | 1.0165–0.1078X | 2 | |
| SP3 | F | 18 | 0.91 | 53 | 0.0250 | 53 | 9.33 | 0.994 | 1.55 | 0.957 | ||
| I | 17 | 0.91 | 11 | 0.0055 | 11 | −0.21 | 0.510 | 1.66 | 0.963 | 0.9724–0.0385X | 5 | |
| SP4 | F | 14 | 0.79 | 16 | 0.0071 | 16 | 4.80 | 0.972 | −0.96 | 0.168 | ||
| I | 11 | 0.67 | 4 | 0.0018 | 4 | −0.68 | 0.427 | −0.87 | 0.206 | 0.9844–0.0339X | 3 | |
| SP5 | F | 13 | 0.90 | 15 | 0.0069 | 15 | 2.18 | 0.840 | 0.01 | 0.556 | ||
| I | 10 | 0.84 | 4 | 0.0020 | 4 | −0.60 | 0.416 | 0.01 | 0.546 | 0.9399 + 0.0413X | 3 | |
| Total (5 subpopulations) | F | 105 | 0.97 | 72 | 0.0328 | 72 | −1.64 | 0.483 | −0.55 | 0.349 | ||
| I | 76 | 0.96 | 13 | 0.0080 | 13 | −27.05 | 0.001 | −0.54 | 0.339 | 0.9773–0.0401X | 13 | |
F: finite population genetic variance estimates; I: infinite population genetic variance estimates; N: number of multilocus genotypes; Hd: gene diversity; k: mean number of pairwise differences; Pi: nucleotide diversity (average over loci); Theta (k): gene variance based on mean number of pairwise differences; Fs/D (Obs): observed value of Fu’s/Tajima’s statistic testing selective neutrality based on allele frequency; P (Fs/D ≤ Obs): probability of obtaining Fs/D value equal or lower than the observed; LD (|D'|): linkage disequilibrium between sites and X is the nucleotide distance (measured in kilobases; kb); Rm: minimum number of recombination events.