Fig 4. Gapdh, Pdha, Ogdh and Sdha mRNA expression in gastrocnemius muscle after triheptanoin treatment.
Starting at P35, female wild-type and hSOD1G93A mice were treated with triheptanoin (TRIH) or control (CON) diet until they were sacrificed. Quantitative real time PCR analysis of Gapdh, Pdha1, Ogdh and Sdha of the gastrocnemius muscle of 10 and 25 week old wild-type and hSOD1G93A mice untreated or treated with triheptanoin relative to house keeping genes. N-numbers of each group used in all graphs are indicated in the top bar graphs. The insets above each graph show the p-values for the effects of genotype in two-way ANOVAs, while the effect of diet was p>0.05 for each bar graph. When significant, the results of Bonferroni post tests are shown by stars (* p<0.05, ** p<0.01), showing that decreases of mRNA levels of several enzymes in hSOD1G93A mice were not apparent with triheptanoin treatment.