Fig 5. Relative expression of the anaplerotic genes, pyruvate carboxylase Pcx and glutamic pyruvic transferases Gpt1 and 2.
Starting at P35, female wild-type and hSOD1G93A mice were either treated with triheptanoin (TRIH) or control (CON) diet until they were sacrificed. Gene expresssion is compared in the gastrocnemius muscle of 10 and 25 week old triheptanoin treated vs. untreated wild-type and hSOD1G93A mice relative to housekeeping genes. N-numbers of each group used in all graphs are indicated in the top bar graphs. The insets above each graph show the p-values for the effects of genotype in two-way ANOVAs, while the effect of diet was p>0.05 for each bar graph. When significant, the results of Bonferroni post tests are shown by a star (* p<0.05), indicating that triheptanoin treatment prevented the decrease of expression in Gpt2 mRNA.