Fig 6. Gene expression of enzymes involved in the propionyl-CoA carboxylase pathway.
Starting at P35, female wild-type and hSOD1G93A mice were treated with triheptanoin (TRIH) or control (CON) diet until they were sacrificed. Relative expression of the α (Pcca) and β (Pccb) subunits of propionyl-CoA carboxylase and methylmalonyl mutase (Mut). Expresssion is compared in the gastrocnemius muscle of 10 and 25 week old wild-type and hSOD1G93A mice untreated or treated with triheptanoin relative to housekeeping genes. N-numbers used for each group throughout the experiments are indicated in the top bar graphs. The insets above each graph show the p-values for the effects of genotype in two-way ANOVAs, while the effect of diet was p>0.05 for each bar graph. When significant, the results of Bonferroni post tests are indicated by a star (* p<0.05), indicating that triheptanoin treatment in hSOD1G93A mice protected against lowered expression of Pccb and Mut mRNA.