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. 2016 Aug 26;11(8):e0161898. doi: 10.1371/journal.pone.0161898

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© 2016 Williams et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — 12 μm PFA fixed sections were taken of mouse retinas of indicated genotypes at 12 months. Sections were stained for C3b/iC3b/C3c (red) and nuclei (blue). Z-stacks were imaged using confocal microscopy and merged to form a maximum intensity projection (MIP). MIPs were merged with digital interference contrast and arrows highlight the presence of C3 breakdown products in RPE/Bruch’s. Scale bars = 50 μm. Images are representative of n = 6.