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. 2016 Aug 26;11(8):e0161875. doi: 10.1371/journal.pone.0161875

Fig 2. Expression levels of matrix metalloproteinase (MMP) mRNA and protein secretion induced by HAoligo treatment.

Fig 2

RSFs were treated with various concentrations of HAoligos (0–500 μg/ml). A, MMP-1 and MMP-3 mRNA expression levels after 12 or 24 hours of HAoligo treatment as detected by quantitative real-time RT-PCR. Results shown are fold change in mRNA copy number. Values are mean ± S.D. of three independent experiments analyzed in triplicate, and are expressed as n-fold increase compared to untreated control. *p<0.05 and **p<0.01 vs. control. B, MMP-1 and MMP-3 protein secretion in conditioned media after 24 hours of HAoligo treatment as evaluated by immunoblot analysis. The amount of sample separated by SDS-PAGE before transfer and immunostaining was determined by the DNA content of RSFs. Results shown are representative of three experiments with similar results. C, The activity of MMPs secreted into conditioned media was evaluated by casein zymography. RSFs were stimulated with 0, 100, or 250 μg/ml HAoligos for 24 hours. The image was digitally inverted to generate dark bands on a light background.