Fig 4. Intracellular signaling cascades stimulated by HAoligos with pre- and co-incubation with a TLR4 neutralizing antibody.

RSFs were pre-treated for one hour with an anti-TLR4 antibody, followed by HAoligo treatment (250 μg/ml). Phosphate buffered saline was substituted as a control. A, Phospho-nuclear factor κB (NF-κB), NF-κB, phospho-p38 mitogen-activated protein kinase (MAPK), p38 MAPK, phospho-Erk, Erk, phospho-c-Jun N-terminal kinase (JNK), and JNK, as evaluated by immunoblot analysis of RSFs derived from three patients with RA. Results shown are representative of three experiments with similar results. B, Densitometry of phospho-NF-κB/NF-κB, phospho-p38 MAPK/p38 MAPK, phospho-ERK/ERK and phospho-JNK/JNK. Values are mean ± S.D. of three independent experiments and expressed as n-fold increase compared to untreated control at time 0. *p<0.05 and **p<0.01 vs. control.