Appendix 2.
Genetic properties of 18 SSR markers developed by Witherup et al. (2013) tested on Artocarpus altilis and congeners.
| Diploid A. altilis (n = 33) | A. camansi (n = 1) | A. heterophyllus (n = 1) | ||||||||||||||||||||
| Vanuatu (n = 27) | New Caledonia (n = 6) | Triploid A. altilis (n = 6) | ||||||||||||||||||||
| Locus | Obs | A | Allele size range (bp)a | Ho | He | HWEb | Obs | A | Allele size range (bp)a | Ho | He | Obs | A | Allele size range (bp)a | % Hetc | IndDisd | A | Allele size range (bp)a | A | Allele size range (bp)a | PIC | |
| MAA3 | 26 | 1 | 234 | 0.000 | 0.000 | — | 5 | 1 | 234 | 0.000 | 0.000 | 6 | 1 | 234 | 0.0 | 0/0/6 | 1 | 234 | 1 | 236 | 0.05 | |
| MAA40 | 26 | 6 | 195–210 | 0.875 | 0.810 | * | 5 | 2 | 208–210 | 0.800 | 0.461 | 6 | 3 | 201–210 | 100.0 | 0/6/0 | 1 | 199 | 1 | 189 | 0.81 | |
| MAA54a | 26 | 8 | 191–212 | 0.813 | 0.819 | ns | 5 | 5 | 191–212 | 1.000 | 0.700 | 4 | 5 | 191–212 | 75.0 | 0/3/1 | 1 | 196 | 1 | 181 | 0.83 | |
| MAA54b | 25 | 3 | 222–232 | 0.613 | 0.613 | ns | 5 | 2 | 222–224 | 0.800 | 0.461 | 4 | 3 | 220–232 | 75.0 | 1/2/1 | 1 | 224 | 1 | 217 | 0.57 | |
| MAA71 | 26 | 9 | 172–195 | 0.875 | 0.779 | ns | 5 | 5 | 180–198 | 1.000 | 0.669 | 5 | 6 | 170–195 | 100.0 | 2/3/0 | 1 | 174 | 2 | 142–183 | 0.79 | |
| MAA85 | 22 | 5 | 174–187 | 0.679 | 0.707 | ns | 5 | 3 | 178–187 | 0.200 | 0.385 | 5 | 3 | 174–179 | 40.0 | 0/2/3 | — | — | — | — | 0.69 | |
| MAA96 | 23 | 7 | 220–230 | 0.310 | 0.727 | * | 5 | 4 | 220–228 | 0.800 | 0.585 | 6 | 5 | 222–230 | 83.3 | 0/5/1 | 1 | 226 | — | — | 0.76 | |
| MAA122 | 25 | 7 | 297–311 | 0.774 | 0.717 | ns | 5 | 3 | 305–311 | 1.000 | 0.607 | 5 | 4 | 297–309 | 100.0 | 0/5/0 | 1 | 299 | 1 | 272 | 0.73 | |
| MAA135 | 24 | 9 | 287–312 | 0.900 | 0.806 | ns | 5 | 4 | 289–308 | 1.000 | 0.622 | 4 | 4 | 287-308 | 100.0 | 3/1/0 | 1 | 318 | 1 | 370 | 0.81 | |
| MAA140 | 27 | 8 | 150–179 | 0.545 | 0.774 | * | 6 | 4 | 150–169 | 0.833 | 0.586 | 6 | 3 | 165–179 | 83.3 | 2/3/1 | 1 | 159 | 1 | 151 | 0.78 | |
| MAA145 | 23 | 6 | 294–323 | 0.679 | 0.690 | * | 5 | 4 | 294–308 | 0.800 | 0.632 | 5 | 5 | 305–323 | 100.0 | 2/3/0 | 1 | 286 | 1 | 294 | 0.71 | |
| MAA156 | 27 | 10 | 290–319 | 0.697 | 0.699 | ns | 6 | 4 | 290–311 | 1.000 | 0.680 | 6 | 4 | 295–324 | 83.3 | 5/0/1 | 1 | 292 | 1 | 292 | 0.72 | |
| MAA182 | 27 | 8 | 197–230 | 0.303 | 0.566 | * | 6 | 1 | 224 | 0.000 | 0.000 | 6 | 3 | 221–227 | 50.0 | 0/3/3 | 1 | 226 | 1 | 191 | 0.53 | |
| MAA201 | 26 | 4 | 281–300 | 0.406 | 0.573 | ns | 6 | 2 | 281–282 | 0.000 | 0.231 | 6 | 7 | 281–311 | 83.3 | 2/3/1 | 1 | 294 | 2 | 258–282 | 0.53 | |
| MAA219 | 24 | 4 | 279–291 | 0.733 | 0.607 | ns | 5 | 3 | 279–300 | 1.000 | 0.561 | 6 | 3 | 279–291 | 50.0 | 0/3/3 | 1 | 294 | 1 | 279 | 0.57 | |
| MAA251 | 26 | 3 | 190–208 | 0.625 | 0.536 | ns | 6 | 2 | 190–208 | 0.000 | 0.231 | 5 | 5 | 190–217 | 100.0 | 2/3/0 | 1 | 214 | 1 | 211 | 0.49 | |
| MAA287 | 27 | 7 | 204–241 | 0.242 | 0.683 | * | 6 | 1 | 212 | 0.000 | 0.000 | 6 | 5 | 200–228 | 83.3 | 3/2/1 | 1 | 196 | 2 | 196–228 | 0.63 | |
| MAA293 | 27 | 4 | 176–188 | 0.727 | 0.616 | ns | 6 | 3 | 176–184 | 1.000 | 0.590 | 6 | 3 | 178–184 | 83.3 | 0/5/1 | 1 | 180 | 1 | 184 | 0.56 | |
Note: A = number of alleles; He = expected heterozygosity; Ho = observed heterozygosity; HWE = Hardy–Weinberg equilibrium; Obs = number of observations; PIC = polymorphism information content value, calculated on diploid data, as described by Botstein et al. (1980).
a
Including the 19-bp M13-tail.
b
To test HWE, a χ2 goodness-of-fit method was used; * = significant at P < 0.05, ns = no significant deviation from HWE.
c
Due to allele ambiguity in the triploids, the He:Ho ratio is replaced by the percentage of heterozygous genotypes.
d
Number of triploid individuals with three, two, or one allele(s).