Figure 4.

Histological observations of bone regeneration in cranial bone defects in rats: HE and MT staining. The periosteal side was on the top, while the dura side was at the bottom. In HE, bone tissue was stained in red and residual materials (mainly CPC) were stained in purple or pink. In MT, the bone tissue was stained in deep blue or red, the residual materials were stained in light blue or white, and the collagen fibers were blue-stained fibrous structures. (I) and (J) were high magnification images of typical new bone structure within the defects of the dotted-line rectangle areas in (E) and (F), respectively.