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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: J Trauma Acute Care Surg. 2016 Sep;81(3):512–519. doi: 10.1097/TA.0000000000001137

Figure 4.

Figure 4

Tubastatin-A protects Caco-2 cells against anoxia (12 hours) injury. Anoxia condition caused significant increased leakage of LDH (*control vs. sham, P<0.0001, 4A), while incubation with Tubastatin-A significantly attenuated the increase (*Tub-A vs. control, P<0.0001, 4A). MTT assay showed that anoxia had a significant decrease of cellular viability compare to sham cells (*control vs. sham, P<0.0001, 3B), while Tubastatin-A treatment significantly restored cellular viability (*Tub-A vs. control, P<0.0001, Figure 4B, 4C). Tub-A: Tubastatin-A treatment; Sham: no anoxia and no treatment; Control: anoxia without treatment; N.C.: negative control.