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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2016 Jul 14;36(9):1821–1828. doi: 10.1161/ATVBAHA.116.307639

Figure 1. 5G6 Fab inhibits GPIbα shedding of stored platelets.

Figure 1

(A) Overview of the LR-ADP storage study. (B) Binding of Fab to stored LR-ADP. Binding was detected by flow cytometry using FITC-conjugated goat anti-mouse IgG, and quantitated by mean fluorescence intensity (MFI). (C) The release of glycocalicin (GC) during storage. The GC level in the supernatant of LR-ADP was detected by Western blot using WM23. The blot shown in the top panel is a representative of 5 independent experiments. The intensities of GC bands in the same blot were quantified and plotted as the fold change over the GC level in the 2-day-old LR-ADP before storage. (D) The surface expression level of GPIbα in LR-ADP during storage was assessed by flow cytometry using biotinylated WM23 and FITC-conjugated streptavidin. The measured MFI was normalized with the level of GPIbα in 2-day-old LR-ADP as 100%. (E) Overview of the hTg platelet storage study. (F) Binding of Fab to stored hTg platelets. (G, H) GPIbα and GPVI surface expression level in stored hTg platelets were quantified using WM23 or JAQ-1 antibody respectively. Results are shown as mean ± SEM (n = 5). **, P < 0.01; *, P < 0.05 (t test). Note: in some case the curve of saline was partially obscured by that of Ctrl Fab.