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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2016 Jul 14;36(9):1821–1828. doi: 10.1161/ATVBAHA.116.307639

Figure 2. 5G6 Fab does not alter the function of stored platelets.

Figure 2

LR-ADP and hTg PRP were stored with saline, Ctrl Fab or 5G6 Fab, then were stimulated with different agonists, and aggregation was measured. (A) LR-ADP aggregation traces are shown. (B-E) The extents of maximal aggregation are plotted versus the age of stored LR-ADP. Stored LR-ADP were stimulated by 1.25 mg/ml ristocetin (B), 100 mM ADP (C), 20 mg/ml collagen (D) or 10 mM ADP + 10 mg/ml Collagen (E). (F–I) The aggregation trace of stored hTg PRP was recorded. After storage for 16 hours, hTg PRP were stimulated with different agonists 10 µM ADP (F), 10 µg/ml collagen (G), 0.4 µg/ml (H) or 0.2 µg/ml (I) botrocetin, and light transmission was recorded. Data are shown as mean ± SEM (n=4).