Skip to main content
. Author manuscript; available in PMC: 2016 Aug 31.
Published in final edited form as: Cell Rep. 2016 Aug 11;16(8):2142–2155. doi: 10.1016/j.celrep.2016.07.055

Figure 6. Coupling motors to mitochondria during cell division causes mitochondrial asymmetry.

Figure 6

(A–C) HeLa cells transiently expressing Tom20-mCherry-FKBP and either HA-BICD2-FRB or HA-KIF5B MD-FRB were treated with ethanol (control) or rapalog at G2, prometaphase, or metaphase and imaged by confocal microscopy at metaphase. The asymmetric index of metaphase cells was calculated on 3D projections of treated cells for BICD-FRB transfected cells (B) and KIF5B–FRB transfected cells (C). (D–F) HeLa cells transfected as in (A) were imaged during telophase. An asymmetric index of the two daughters cells mitochondrial content was calculated for cells expressing BICD-FRB (E) or KIF5B–FRB (F). Data are represented as median with the interquartile range. Scale bars represent 5 microns. *p < 0.05, **p < 0.01, ***p < 0.001.