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. 2016 Mar 14;34(11):1894–1904. doi: 10.1002/jor.23216

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© 2016 The Authors. Journal of Orthopaedic Research published by Wiley Periodicals, Inc. on behalf of the Orthopaedic Research Society.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Expression of MMP9 active and inactive forms within the area of HO. Representative photomicrographs of paraffin sections serially cut from rat (A), mouse (B), or human (C) tissues. Rat and mouse tissues were isolated 2, 4, and 6 days after delivery of AdBMP2 transduced cells. Human tissues depict soft tissues immediately surrounding the newly forming heterotopic bone. Tissue sections were stained with hematoxylin and eosin or co‐immunostained with antibodies that detect total MMP9 (red) or the active form (green), or for human tissues total MMP9 (green) and active form (red) as indicated.