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. 2016 Mar 14;34(11):1894–1904. doi: 10.1002/jor.23216

Figure 5.

Figure 5

Quantification of MMP9 protein and functional activity by ELISA. Tissues were isolated from rats at 2, 4, and 6 days after proximal injection of AdBMP2 and AdEmpty transduced cells and protein extracts generated. (A) MMP9 protein was bound through ELISA, and then substrate added, to quantify MMP9 functional activity. (B) MMP9 total protein was bound through ELISA and quantified directly. Standard amounts of active MMP9 or total MMP9 protein respectively were assayed in order to calculate active protein amount from the activity measurements. Statistical significance was calculated using a standard t‐test, with n = 6. *Denotes statistical significance. Sample comparisons between the various days were calculated using a paired one‐way ANOVA with Tukey post‐hoc correction for multiple comparisons with 95% confidence interval (p < 0.05). A student t‐test was used to determine significance between the treated and control groups.