Fig. 2.

Schematic diagram of midbrain dopaminergic neuron differentiation. The differentiation of midbrain dopaminergic neurons from pluripotent stem cell colonies is driven by the transition from spherical to adherent cultures and a staggered supplementation of growth factors, particularly SHH, FGF8 and later TGF3β. The presence or absence of developmental and maturation markers are essential guides to monitor the differentiation progress at each culturing stage towards mature, functional midbrain dopaminergic neurons