Table 3.
Comparison of astrocyte differentiation protocols
| Differentiation protocol | Krencik et al. [117] | Emdad et al. [118] | Serio et al. [119] | Shaltouki et al. [120] | Roybon et al. [122] | Mormone et al. [121] |
|---|---|---|---|---|---|---|
| Duration (days) | 120 | 35 | 49 | 42 | 90 | 35 |
| Efficiency (GFAP+ cells) | 90 % | 70 % | 90 % | 70 % | 70 % | 55 % (without sorting) |
| Growth factors | CNTF (or LIF), EGF, FGF2 | CNTF, EGF, FGF2 | CNTF, EGF, FGF2 LIF | CNTF, FGF2, NRG1β1 | AA, BDNF, CNTF, FGF2, GDNF, IGF, RA; maturation induced by withdrawal of growth factors | CNTF, EGF, FGF2 |
| Developmental markers (protein, mRNA) | CD44, GFAP, NF1A | GFAP | GFAP, NF1A, vimentin | CD44, GFAP, NF1A | AQP4, CD44, GFAP, NF1A, S100β, vimentin | GFAP |
| Maturity markers (protein, mRNA) | S100β | AQP4, EAAT1 | EAAT1, S100β | ALDOC, EAAT1, S100β | ALDH1L1, EAAT1, EAAT2 | ALDOC, EAAT2 |
| Physiological function | Propagation of Ca2+ waves, glutamate uptake | Migratory properties | Glutamate uptake, promotion of synaptogenesis in neuron co-cultures | Glutamate uptake, promotion of synaptogenesis in neuron co-cultures | Propagation of Ca2+, glutamate uptake, inflammatory response (IL-6 release) | Migratory properties |