Extracellular ion transport pathways in TWIK-1. (A) Side view (upper panel) and top view (bottom panel) of the region between the EC (G80) and the SF (G121), where the position of K+ ions (orange spheres) was tracked. (B–G) Color maps showing the position of extracellular ions entering the filter region in simulations HSE, HSD, HSP, HSEP, HSDP, and HSPP, respectively. Ion positions were measured as the x and y coordinates of the center of mass and then discretized into bins of 0.5 Å. To see this figure in color, go online.