Figure 1.

Experimental setup. Twelve populations were entrained under staggered 12 h:12 h L/D regimes and combined into a single experiment. A multiplexed measurement of phase shift or growth rate modulation was achieved by exposing the mixed-phase population to a single pulse of darkness (scale bar, 5 μm). Fluorescence and brightfield micrographs recorded every hour were used to extract every cell’s physiological parameters (e.g., length and clock reporter). To see this figure in color, go online.