Figure 3.

Representative time-resolved fluorescence anisotropy decays of single-Trp mutants of α-synuclein. (A) Trp-39. (B) Trp-78. (C) Trp-140. The insets show the global fitting of parallel (I_||(t)) and perpendicular (I_⊥(t)) intensity components. (D) Trp-107. The inset shows the overlay of the decay fits for Trp-78 and Trp-107 in the log scale, indicating a slower decay for Trp-107. The fits are indicated by black solid lines. See Materials and Methods for data acquisition and analysis and Table S1 for the mean fluorescence lifetime and the typical parameters associated with the decay analysis. See Figs. S3–S6 for the global fitting of parallel and perpendicular intensity decays, residual plots, and the autocorrelation analysis. A representative plot of rotational correlation time (ϕ_slow) and goodness of fit (χ²), indicating the robustness of the recovered rotational correlation times, is shown in Fig. S7. To see this figure in color, go online.