Table 1.
Haematological parameters of Case 1 and Case 2 measured during clinical EEHV-1A infection
| A | |||||||||
| Days | WBC (*10e9/L) | Neutrophils (*10e9/L) | Lymphocytes (*10e9/L) | Monocytes (*10e9/L) | Eosinophils (*10e9/L) | Basophils (*10e9/L) | |||
| 7 | 22.85 | 7.77 | 8.91 | 5.94 (26 %) | 0.23 | 0 | |||
| 23 | 13.69 | 5.75 | 2.87 | 6.16 (45 %) | 0 | 0 | |||
| 213 | 15.91 | 4.61 | 5.73 | 5.25 (33 %) | 0.32 | 0 | |||
| Physiological mean (% of WBC) |
15.51 | 3.77 (25.5 %) | 5.02 (23.65 %) |
6.46 (50 %) |
0.24 (1.55 %) |
0.02 (0.10 %) |
|||
| Days | RBC (*10e12/L) | HGB (g/dL) | HCT (%) | MCV (fL) | MCH (pg) | MCHV (g/dL) | RDW (%) |
PLT (*10e9/L) | PCV (%) |
| 7 | 3.54 | 13.4 | 37.7 | 107 | 37.8 | 35.5 | 14.3 | 470 | 36 |
| 23 | 5.06 | 19.2 | 55.1 | 109 | 37.9 | 34.8 | 14.5 | 968 | 55 |
| 213 | 3.62 | 13.3 | 39.2 | 108 | 36.8 | 33.9 | 13.9 | 996 | 37 |
| Physiological (mean) | 3.55 | 14.28 | 40.60 | 114.25 | 40.19 | 35.17 | 14.33 | 617.65 | 40.80 |
| B | |||||||||
| Days | EEHV-1A (vgc/ml) |
WBC (*10e9/L) |
Neutrophils (*10e9/L) | Lymphocytes (*10e9/L) | Monocytes (*10e9/L) | Eosinophils (*10e9/L) | Basophils (*10e9/L) | ||
| -33 | 5.0 × 102 | 23.08 | 4.62 | 5.77 | 12.46 (54 %) | 0 | 0 | ||
| -29 | ND | 25.34 | 4.56 | 7.6 | 13.18 (52) % | 0 | 0 | ||
| -5 | 1.2 × 105 | 6.18 | 3.46 | 1.85 | 0.8 (13 %) | 0.06 | 0 | ||
| -3 | 2.7 × 106 | 7.35 | 5.22 | 1.32 | 0.81 (11 %) | 0 | 0 | ||
| 0 | 7.2 × 106 | 13.25 | 8.22 | 2.65 | 2.25 (17 %) | 0.13 | 0 | ||
| 1 | 1.9 × 107 | 12.925 | 7.565 | 2 | 2.975 (23 %) | 0 | 0.195 | ||
| Physiological mean (% of WBC) | 15.51 | 3.77 (25.5 %) | 5.02 (23.65 %) | 6.46 (50 %) | 0.24 (1.55 %) | 0.02 (0.10 %) | |||
| Days | RBC (*10e12/L) | HGB (g/dL) | HCT (%) | MCV (fL) | MCH (pg) | MCHV (g/dL) | RDW (%) | PLT (*10e9/L) | PCV (%) |
| -33 | 3.65 | 14.6 | 43.2 | 118.3 | 39.9 | 33.7 | 13.9 | 950 | 42 |
| -29 | 3.58 | 14.5 | 41.4 | 115.5 | 40.4 | 35 | 38.1 | 760 | 38 |
| -5 | 3.48 | 14.2 | 41.8 | 120.4 | 41 | 34 | 14 | 560 | 37 |
| -3 | 3.36 | 14.2 | 38.4 | 114.4 | 42.4 | 37.1 | 13.7 | 183 | 38 |
| 0 | 2.89 | 12.4 | 32.9 | 113.7 | 42.9 | 37.8 | 13.8 | 98 | 34 |
| 1 | 3.07 | 12.95 | 35.05 | 114.2 | 42.2 | 36.9 | 13.6 | 13 | 36.5 |
| Physiological mean | 3.55 | 14.28 | 40.60 | 114.25 | 40.19 | 35.17 | 14.33 | 617.65 | 40.80 |
The haematological parameters for Case 1 (A) were only available for Days 7, 23 and 213 following suspicion of clinical EEHV-1A infection. The haematological parameters and viral load for Case 2 (B) were measured on several occasions from 33 days prior to the observation of signs indicative of clinical EEHV-1A infection (Day 0) to the Day 1 when the elephant succumbed to the infection at midnight. Normal platelet range of live juveniles in the collection was established, during a period of seven months from 20 blood samples of the elephants, to be at 372-878*10e9/L (mean of 625*10e9/L). The rest of the physiological parameters were from 24 measurements from four individuals under eight years of age (age range at time of sampling: 1.5-7y). Parameter values with a substantial change are highlighted in bold. Percentages of monocytes in the white blood cells are also given in the table
vgc/ml, viral genome copies per millilitre of EDTA whole blood; WBC, white blood cell count; RBC, red blood cell count; HGB, haemoglobin; HCT, haematocrit; MCV, mean cell volume; MCH, mean corpuscular haemoglobin; MCHV, mean corpuscular haemoglobin volume; RDW, red blood cell distribution width; PLT, platelet count; PCV, packed cell volume; ND, not detected