Table 1.
Primer sequences
| Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|
| CD11b | GAACATCCCATGACCTTCCA | GCTGGGGGACAGTAGAAACA |
| CD11c | ACGTCAGTACAAGGAGATGTTGGA | ATCCTATTGCAGAATGCTTCTTTACC |
| CD68 | CTTCCCACAGGCAGCACAG | AATGATGAGAGGCAGCAAGAGG |
| Claudin 1 | TTCGCAAAGCACCGGGCAGATACA | GCCACTAATGTCGCCAGACCTGAAA |
| Claudin 5 | GTTAAGGCACGGGTAGCACT | GTACTTCTGTGACACCGGCA |
| COX-2 | TGACCCCCAAGGCTCAAATAT | TGAACCCAGGTCCTCGCTTA |
| F4/80 | TGACAACCAGACGGCTTGTG | CAGGCGAGGAAAAGATAG |
| GFAP | TTCGCACTCAATACGAGGCA | CTCCAGATCGCAGGTCAAG |
| IL-1β | TCGCTCAGGGTCACAAGAAA | CATCAGAGGCAAGGAGGAAAAC |
| IL-6 | ACAAGTCGGAGGCTTAATTACACAT | TTGCCATTGCACAACTCTTTTC |
| iNOS | AGGTACTCAGCGTGCTCCAC | GCACCGAAGATATCTTCATG |
| LBP | AGTCCTGGGAATCTGTCCTTG | ACTTGTGCCTTGTCTGGATG |
| MCP-1 | GCAGTTAACGCCCCACTCA | TCCAGCCTACTCATTGGGATCA |
| Occludin | ATGTCCGGCCGATGCTCTC | TTTGGCTGCTCTTGGGTCTGTAT |
| RPL19 | TGACCTGGATGAGAAGGATGAG | CTGTGATACATATGGCGGTCAATC |
| Serpina3n | GGACATTGATGGTGCTGGTGAAT | CTCCTCTTGCCCGCGTAGAA |
| TNFα | CCACCACGCTCTTCTGTCT | TCCAGCTGCTCCTCCACTT |
| ZO-1 | TTTTTGACAGGGGGAGTGG | TGCTGCAGAGGTCAAAGTTCAAG |