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. 2016 Aug 26;17(1):684. doi: 10.1186/s12864-016-3032-4

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Potential novel miRNAs identified in this study. a The secondary structure of novel_ miR_36 precursor was predicted by MFOLD. Mature miRNA is highlighted in red. b Stem-loop RT-PCR analysis of the identified novel miRNAs. Six novel miRNAs (novel_miR_2, novel_miR_13, novel_miR_29, novel_miR_32, novel_miR_36, novel_miR_66) were confirmed via stem-loop RT-PCR. The sizes of the obtained PCR products were approximately 60 bp. Marker indicates a 50 bp DNA Ladder Marker